High sensitivity optical biosensor for real-time label-free binding kinetics.• robust kinetics determination of off-rates up to 10 s-1 • reliable binding affinities from low pM to low mM • superior signal-to-noise ratios (0.01 pg/mm2 at 1 Hz) • compatible with crude samples, harsh chemicals, and particles up to 1000 nm Includes WAVEcore (device), WAVEsampler (autosampler), WAVEcontrol (2 perpetual software licenses) and: